Construct: shRNA TRCN0000194665

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138316.2-716s1c1
DNA Barcode:
CCTATGTTGCTGGTTAACATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PANK1 (53354)
Gene Description:
pantothenate kinase 1
Transcript:
RefSeq NM_138316.2 (NON-CURRENT)
Match location:
Position 716 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194665)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATGTTGCTGGTTAACATG
Hairpin Sequence:
5'-CCGG-CCTATGTTGCTGGTTAACATG-CTCGAG-CATGTTAACCAGCAACATAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATGTTGCTGGTTAACATGCTCGAGCATGTTAACCAGCAACATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATGTTGCTGGTTAACATGCTCGAGCATGTTAACCAGCAACATAGG-3'

Other clones with same target sequence:

(none)