Construct: shRNA TRCN0000194694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004938.2-4965s1c1
DNA Barcode:
CCTTGCTTCTTACTGATAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK1 (1612)
Gene Description:
death associated protein kinase 1
Transcript:
RefSeq NM_004938.2 (NON-CURRENT)
Match location:
Position 4965 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCTTCTTACTGATAATT
Hairpin Sequence:
5'-CCGG-CCTTGCTTCTTACTGATAATT-CTCGAG-AATTATCAGTAAGAAGCAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCTTCTTACTGATAATTCTCGAGAATTATCAGTAAGAAGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCTTCTTACTGATAATTCTCGAGAATTATCAGTAAGAAGCAAGG-3'

Other clones with same target sequence:

(none)