Construct: shRNA TRCN0000194711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002748.2-2631s1c1
DNA Barcode:
CACTAGTTACTTGGACAAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK6 (5597)
Gene Description:
mitogen-activated protein kinase 6
Transcript:
RefSeq NM_002748.2 (NON-CURRENT)
Match location:
Position 2631 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTAGTTACTTGGACAAGTT
Hairpin Sequence:
5'-CCGG-CACTAGTTACTTGGACAAGTT-CTCGAG-AACTTGTCCAAGTAACTAGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTAGTTACTTGGACAAGTTCTCGAGAACTTGTCCAAGTAACTAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTAGTTACTTGGACAAGTTCTCGAGAACTTGTCCAAGTAACTAGTG-3'

Other clones with same target sequence:

  1. TRCN0000315272