Construct: shRNA TRCN0000194777

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024594.2-445s1c1
DNA Barcode:
CGGAAATATTTGACTTCTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PANK3 (79646)
Gene Description:
pantothenate kinase 3
Transcript:
RefSeq NM_024594.2 (NON-CURRENT)
Match location:
Position 445 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194777)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAATATTTGACTTCTAAC
Hairpin Sequence:
5'-CCGG-CGGAAATATTTGACTTCTAAC-CTCGAG-GTTAGAAGTCAAATATTTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAATATTTGACTTCTAACCTCGAGGTTAGAAGTCAAATATTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAATATTTGACTTCTAACCTCGAGGTTAGAAGTCAAATATTTCCG-3'

Other clones with same target sequence:

  1. TRCN0000308115