Construct: shRNA TRCN0000194783

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002730.3-1707s1c1
DNA Barcode:
CAACCTTCCTTTCGGAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKACA (5566)
Gene Description:
protein kinase cAMP-activated catalytic subunit alpha
Transcript:
RefSeq NM_002730.3 (NON-CURRENT)
Match location:
Position 1707 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194783)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCTTCCTTTCGGAGTAAT
Hairpin Sequence:
5'-CCGG-CAACCTTCCTTTCGGAGTAAT-CTCGAG-ATTACTCCGAAAGGAAGGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCTTCCTTTCGGAGTAATCTCGAGATTACTCCGAAAGGAAGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCTTCCTTTCGGAGTAATCTCGAGATTACTCCGAAAGGAAGGTTG-3'

Other clones with same target sequence:

  1. TRCN0000233528