Construct: shRNA TRCN0000194789

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015092.3-5670s1c1
DNA Barcode:
CCAACTTTACTTGGCAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMG1 (23049)
Gene Description:
SMG1 nonsense mediated mRNA decay associated PI3K related kinase
Transcript:
RefSeq NM_015092.3 (NON-CURRENT)
Match location:
Position 5670 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194789)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACTTTACTTGGCAATATT
Hairpin Sequence:
5'-CCGG-CCAACTTTACTTGGCAATATT-CTCGAG-AATATTGCCAAGTAAAGTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACTTTACTTGGCAATATTCTCGAGAATATTGCCAAGTAAAGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACTTTACTTGGCAATATTCTCGAGAATATTGCCAAGTAAAGTTGG-3'

Other clones with same target sequence:

(none)