Construct: shRNA TRCN0000194829

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173655.1-868s1c1
DNA Barcode:
CCAAACATCATTCGCCTAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA6 (285220)
Gene Description:
EPH receptor A6
Transcript:
RefSeq NM_173655.1 (NON-CURRENT)
Match location:
Position 868 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194829)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACATCATTCGCCTAGAA
Hairpin Sequence:
5'-CCGG-CCAAACATCATTCGCCTAGAA-CTCGAG-TTCTAGGCGAATGATGTTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACATCATTCGCCTAGAACTCGAGTTCTAGGCGAATGATGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACATCATTCGCCTAGAACTCGAGTTCTAGGCGAATGATGTTTGG-3'

Other clones with same target sequence:

(none)