Construct: shRNA TRCN0000194874

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004850.3-922s1c1
DNA Barcode:
CCTTTCAAGATGATAGGTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROCK2 (9475)
Gene Description:
Rho associated coiled-coil containing protein kinase 2
Transcript:
RefSeq NM_004850.3 (NON-CURRENT)
Match location:
Position 922 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194874)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCAAGATGATAGGTATC
Hairpin Sequence:
5'-CCGG-CCTTTCAAGATGATAGGTATC-CTCGAG-GATACCTATCATCTTGAAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCAAGATGATAGGTATCCTCGAGGATACCTATCATCTTGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCAAGATGATAGGTATCCTCGAGGATACCTATCATCTTGAAAGG-3'

Other clones with same target sequence:

(none)