Construct: shRNA TRCN0000194915

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012987.1-151s1c1
DNA Barcode:
CATGGAGGAGTTAACAGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DKFZp686K16132 (388957)
Gene Description:
hypothetical LOC388957
Transcript:
RefSeq NM_001012987.1 (NON-CURRENT)
Match location:
Position 151 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194915)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGAGGAGTTAACAGTTAT
Hairpin Sequence:
5'-CCGG-CATGGAGGAGTTAACAGTTAT-CTCGAG-ATAACTGTTAACTCCTCCATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGAGGAGTTAACAGTTATCTCGAGATAACTGTTAACTCCTCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGAGGAGTTAACAGTTATCTCGAGATAACTGTTAACTCCTCCATG-3'

Other clones with same target sequence:

(none)