Construct: shRNA TRCN0000194940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004080.1-2632s1c1
DNA Barcode:
CCTGAAGTGATACTGCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGKB (1607)
Gene Description:
diacylglycerol kinase beta
Transcript:
RefSeq NM_004080.1 (NON-CURRENT)
Match location:
Position 2632 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGTGATACTGCATATT
Hairpin Sequence:
5'-CCGG-CCTGAAGTGATACTGCATATT-CTCGAG-AATATGCAGTATCACTTCAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGTGATACTGCATATTCTCGAGAATATGCAGTATCACTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGTGATACTGCATATTCTCGAGAATATGCAGTATCACTTCAGG-3'

Other clones with same target sequence:

(none)