Construct: shRNA TRCN0000194946

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012424.2-2269s1c1
DNA Barcode:
CAATGGGACCTACTAAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPS6KC1 (26750)
Gene Description:
ribosomal protein S6 kinase C1
Transcript:
RefSeq NM_012424.2 (NON-CURRENT)
Match location:
Position 2269 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194946)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGGGACCTACTAAGTTTA
Hairpin Sequence:
5'-CCGG-CAATGGGACCTACTAAGTTTA-CTCGAG-TAAACTTAGTAGGTCCCATTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGGGACCTACTAAGTTTACTCGAGTAAACTTAGTAGGTCCCATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGGGACCTACTAAGTTTACTCGAGTAAACTTAGTAGGTCCCATTG-3'

Other clones with same target sequence:

(none)