Construct: shRNA TRCN0000194957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005627.2-1467s1c1
DNA Barcode:
CTGGAAGCTTAGCAATCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK1 (6446)
Gene Description:
serum/glucocorticoid regulated kinase 1
Transcript:
RefSeq NM_005627.2 (NON-CURRENT)
Match location:
Position 1467 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000194957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAAGCTTAGCAATCTTAT
Hairpin Sequence:
5'-CCGG-CTGGAAGCTTAGCAATCTTAT-CTCGAG-ATAAGATTGCTAAGCTTCCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAAGCTTAGCAATCTTATCTCGAGATAAGATTGCTAAGCTTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAAGCTTAGCAATCTTATCTCGAGATAAGATTGCTAAGCTTCCAG-3'

Other clones with same target sequence:

  1. TRCN0000312569