Construct: shRNA TRCN0000195001

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_370878.3-1749s1c1
DNA Barcode:
CCCTGTTAAGTCACCTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PEAK1 (79834)
Gene Description:
pseudopodium enriched atypical kinase 1
Transcript:
RefSeq XM_370878.3 (NON-CURRENT)
Match location:
Position 1749 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195001)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGTTAAGTCACCTAATTT
Hairpin Sequence:
5'-CCGG-CCCTGTTAAGTCACCTAATTT-CTCGAG-AAATTAGGTGACTTAACAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGTTAAGTCACCTAATTTCTCGAGAAATTAGGTGACTTAACAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGTTAAGTCACCTAATTTCTCGAGAAATTAGGTGACTTAACAGGG-3'

Other clones with same target sequence:

  1. TRCN0000088549