Construct: shRNA TRCN0000195051

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004440.2-1296s1c1
DNA Barcode:
CGATGTGACCTACAGAATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPHA7 (2045)
Gene Description:
EPH receptor A7
Transcript:
RefSeq NM_004440.2 (NON-CURRENT)
Match location:
Position 1296 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195051)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATGTGACCTACAGAATATT
Hairpin Sequence:
5'-CCGG-CGATGTGACCTACAGAATATT-CTCGAG-AATATTCTGTAGGTCACATCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATGTGACCTACAGAATATTCTCGAGAATATTCTGTAGGTCACATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATGTGACCTACAGAATATTCTCGAGAATATTCTGTAGGTCACATCG-3'

Other clones with same target sequence:

  1. TRCN0000318440