Construct: shRNA TRCN0000195166

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014791.2-2073s1c1
DNA Barcode:
CCTATCTAGCTGCAAGGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MELK (9833)
Gene Description:
maternal embryonic leucine zipper kinase
Transcript:
RefSeq NM_014791.2 (NON-CURRENT)
Match location:
Position 2073 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195166)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATCTAGCTGCAAGGTATA
Hairpin Sequence:
5'-CCGG-CCTATCTAGCTGCAAGGTATA-CTCGAG-TATACCTTGCAGCTAGATAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATCTAGCTGCAAGGTATACTCGAGTATACCTTGCAGCTAGATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATCTAGCTGCAAGGTATACTCGAGTATACCTTGCAGCTAGATAGG-3'

Other clones with same target sequence:

  1. TRCN0000280031