Construct: shRNA TRCN0000195241

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004103.3-1455s1c1
DNA Barcode:
CATTCAAGGATGGAACATTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTK2B (2185)
Gene Description:
protein tyrosine kinase 2 beta
Transcript:
RefSeq NM_004103.3 (NON-CURRENT)
Match location:
Position 1455 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195241)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTCAAGGATGGAACATTAC
Hairpin Sequence:
5'-CCGG-CATTCAAGGATGGAACATTAC-CTCGAG-GTAATGTTCCATCCTTGAATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTCAAGGATGGAACATTACCTCGAGGTAATGTTCCATCCTTGAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTCAAGGATGGAACATTACCTCGAGGTAATGTTCCATCCTTGAATG-3'

Other clones with same target sequence:

(none)