Construct: shRNA TRCN0000195265
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_014413.2-425s1c1
- DNA Barcode:
- CAGAGCTATTACTCACTTAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- EIF2AK1 (27102)
- Gene Description:
- eukaryotic translation initiation factor 2 alpha kinase 1
- Transcript:
- RefSeq NM_014413.2 (NON-CURRENT)
- Match location:
- Position 425 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000195265)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAGAGCTATTACTCACTTAAT
- Hairpin Sequence:
-
5'-CCGG-CAGAGCTATTACTCACTTAAT-CTCGAG-ATTAAGTGAGTAATAGCTCTG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAGAGCTATTACTCACTTAATCTCGAGATTAAGTGAGTAATAGCTCTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAGAGCTATTACTCACTTAATCTCGAGATTAAGTGAGTAATAGCTCTG-3'
Other clones with same target sequence:
(none)
