Construct: shRNA TRCN0000195293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017433.3-320s1c1
DNA Barcode:
CTTGATCCAATTCACGATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO3A (53904)
Gene Description:
myosin IIIA
Transcript:
RefSeq NM_017433.3 (NON-CURRENT)
Match location:
Position 320 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGATCCAATTCACGATATT
Hairpin Sequence:
5'-CCGG-CTTGATCCAATTCACGATATT-CTCGAG-AATATCGTGAATTGGATCAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGATCCAATTCACGATATTCTCGAGAATATCGTGAATTGGATCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGATCCAATTCACGATATTCTCGAGAATATCGTGAATTGGATCAAG-3'

Other clones with same target sequence:

(none)