Construct: shRNA TRCN0000195330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005781.4-1764s1c1
DNA Barcode:
CCAGATGAATGATGTCATCAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNK2 (10188)
Gene Description:
tyrosine kinase non receptor 2
Transcript:
RefSeq NM_005781.4 (CURRENT)
Match location:
Position 1764 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATGAATGATGTCATCAC
Hairpin Sequence:
5'-CCGG-CCAGATGAATGATGTCATCAC-CTCGAG-GTGATGACATCATTCATCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATGAATGATGTCATCACCTCGAGGTGATGACATCATTCATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATGAATGATGTCATCACCTCGAGGTGATGACATCATTCATCTGG-3'

Other clones with same target sequence:

(none)