Construct: shRNA TRCN0000195335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014586.1-1103s1c1
DNA Barcode:
CCATAGGTGTGAACATGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HUNK (30811)
Gene Description:
hormonally up-regulated Neu-associated kinase
Transcript:
RefSeq NM_014586.1 (NON-CURRENT)
Match location:
Position 1103 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAGGTGTGAACATGTATG
Hairpin Sequence:
5'-CCGG-CCATAGGTGTGAACATGTATG-CTCGAG-CATACATGTTCACACCTATGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAGGTGTGAACATGTATGCTCGAGCATACATGTTCACACCTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAGGTGTGAACATGTATGCTCGAGCATACATGTTCACACCTATGG-3'

Other clones with same target sequence:

(none)