Construct: shRNA TRCN0000195339

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198291.1-2718s1c1
DNA Barcode:
CATCCTCAGGAACCAACAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRC (6714)
Gene Description:
SRC proto-oncogene, non-receptor tyrosine kinase
Transcript:
RefSeq NM_198291.1 (NON-CURRENT)
Match location:
Position 2718 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195339)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCTCAGGAACCAACAATT
Hairpin Sequence:
5'-CCGG-CATCCTCAGGAACCAACAATT-CTCGAG-AATTGTTGGTTCCTGAGGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCTCAGGAACCAACAATTCTCGAGAATTGTTGGTTCCTGAGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCTCAGGAACCAACAATTCTCGAGAATTGTTGGTTCCTGAGGATG-3'

Other clones with same target sequence:

  1. TRCN0000344609