Construct: shRNA TRCN0000195387

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002958.2-994s1c1
DNA Barcode:
CGGATAGAGAAGAACGACTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RYK (6259)
Gene Description:
receptor like tyrosine kinase
Transcript:
RefSeq NM_002958.2 (NON-CURRENT)
Match location:
Position 994 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195387)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATAGAGAAGAACGACTTG
Hairpin Sequence:
5'-CCGG-CGGATAGAGAAGAACGACTTG-CTCGAG-CAAGTCGTTCTTCTCTATCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATAGAGAAGAACGACTTGCTCGAGCAAGTCGTTCTTCTCTATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATAGAGAAGAACGACTTGCTCGAGCAAGTCGTTCTTCTCTATCCG-3'

Other clones with same target sequence:

(none)