Construct: shRNA TRCN0000195434

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003390.2-1859s1c1
DNA Barcode:
CCGGATTCTTTGTTGCTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WEE1 (7465)
Gene Description:
WEE1 G2 checkpoint kinase
Transcript:
RefSeq NM_003390.2 (NON-CURRENT)
Match location:
Position 1859 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195434)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGATTCTTTGTTGCTTCAT
Hairpin Sequence:
5'-CCGG-CCGGATTCTTTGTTGCTTCAT-CTCGAG-ATGAAGCAACAAAGAATCCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGATTCTTTGTTGCTTCATCTCGAGATGAAGCAACAAAGAATCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGATTCTTTGTTGCTTCATCTCGAGATGAAGCAACAAAGAATCCGG-3'

Other clones with same target sequence:

(none)