Construct: shRNA TRCN0000195452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004635.3-2179s1c1
DNA Barcode:
CATTAACCTGAGGTCTCTTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPKAPK3 (7867)
Gene Description:
MAPK activated protein kinase 3
Transcript:
RefSeq NM_004635.3 (NON-CURRENT)
Match location:
Position 2179 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTAACCTGAGGTCTCTTTC
Hairpin Sequence:
5'-CCGG-CATTAACCTGAGGTCTCTTTC-CTCGAG-GAAAGAGACCTCAGGTTAATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTAACCTGAGGTCTCTTTCCTCGAGGAAAGAGACCTCAGGTTAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTAACCTGAGGTCTCTTTCCTCGAGGAAAGAGACCTCAGGTTAATG-3'

Other clones with same target sequence:

  1. TRCN0000279765