Construct: shRNA TRCN0000195477

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003565.1-1064s1c1
DNA Barcode:
CGCATGGACTTCGATGAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ULK1 (8408)
Gene Description:
unc-51 like autophagy activating kinase 1
Transcript:
RefSeq NM_003565.1 (NON-CURRENT)
Match location:
Position 1064 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195477)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATGGACTTCGATGAGTTT
Hairpin Sequence:
5'-CCGG-CGCATGGACTTCGATGAGTTT-CTCGAG-AAACTCATCGAAGTCCATGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATGGACTTCGATGAGTTTCTCGAGAAACTCATCGAAGTCCATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATGGACTTCGATGAGTTTCTCGAGAAACTCATCGAAGTCCATGCG-3'

Other clones with same target sequence:

  1. TRCN0000342594