Construct: shRNA TRCN0000195542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014326.3-728s1c1
DNA Barcode:
CAGGAAACACTGGCAAATATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAPK2 (23604)
Gene Description:
death associated protein kinase 2
Transcript:
RefSeq NM_014326.3 (NON-CURRENT)
Match location:
Position 728 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAAACACTGGCAAATATC
Hairpin Sequence:
5'-CCGG-CAGGAAACACTGGCAAATATC-CTCGAG-GATATTTGCCAGTGTTTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAAACACTGGCAAATATCCTCGAGGATATTTGCCAGTGTTTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAAACACTGGCAAATATCCTCGAGGATATTTGCCAGTGTTTCCTG-3'

Other clones with same target sequence:

  1. TRCN0000352678