Construct: shRNA TRCN0000195589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018032.3-182s1c1
DNA Barcode:
CCAGACAGAGGGTCAAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LUC7L (55692)
Gene Description:
LUC7 like
Transcript:
RefSeq NM_018032.3 (NON-CURRENT)
Match location:
Position 182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGACAGAGGGTCAAGTTTA
Hairpin Sequence:
5'-CCGG-CCAGACAGAGGGTCAAGTTTA-CTCGAG-TAAACTTGACCCTCTGTCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGACAGAGGGTCAAGTTTACTCGAGTAAACTTGACCCTCTGTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGACAGAGGGTCAAGTTTACTCGAGTAAACTTGACCCTCTGTCTGG-3'

Other clones with same target sequence:

(none)