Construct: shRNA TRCN0000195601

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000294.1-1345s1c1
DNA Barcode:
GAACTCTCCAGAAGAAGGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHKG2 (5261)
Gene Description:
phosphorylase kinase catalytic subunit gamma 2
Transcript:
RefSeq NM_000294.1 (NON-CURRENT)
Match location:
Position 1345 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195601)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTCTCCAGAAGAAGGGTT
Hairpin Sequence:
5'-CCGG-GAACTCTCCAGAAGAAGGGTT-CTCGAG-AACCCTTCTTCTGGAGAGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTCTCCAGAAGAAGGGTTCTCGAGAACCCTTCTTCTGGAGAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTCTCCAGAAGAAGGGTTCTCGAGAACCCTTCTTCTGGAGAGTTC-3'

Other clones with same target sequence:

(none)