Construct: shRNA TRCN0000195652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032017.1-1466s1c1
DNA Barcode:
CCAAATGAGCAATGCGGATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK40 (83931)
Gene Description:
serine/threonine kinase 40
Transcript:
RefSeq NM_032017.1 (NON-CURRENT)
Match location:
Position 1466 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATGAGCAATGCGGATAG
Hairpin Sequence:
5'-CCGG-CCAAATGAGCAATGCGGATAG-CTCGAG-CTATCCGCATTGCTCATTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATGAGCAATGCGGATAGCTCGAGCTATCCGCATTGCTCATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATGAGCAATGCGGATAGCTCGAGCTATCCGCATTGCTCATTTGG-3'

Other clones with same target sequence:

(none)