Construct: shRNA TRCN0000195725

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145203.2-812s1c1
DNA Barcode:
CCGAGACATTAAACCAGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1A1L (122011)
Gene Description:
casein kinase 1 alpha 1 like
Transcript:
RefSeq NM_145203.2 (NON-CURRENT)
Match location:
Position 812 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195725)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGACATTAAACCAGATAA
Hairpin Sequence:
5'-CCGG-CCGAGACATTAAACCAGATAA-CTCGAG-TTATCTGGTTTAATGTCTCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGACATTAAACCAGATAACTCGAGTTATCTGGTTTAATGTCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGACATTAAACCAGATAACTCGAGTTATCTGGTTTAATGTCTCGG-3'

Other clones with same target sequence:

(none)