Construct: shRNA TRCN0000195768

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017891.2-1794s1c1
DNA Barcode:
CTCTGGATCTCAGCTGGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C1orf159 (54991)
Gene Description:
chromosome 1 open reading frame 159
Transcript:
RefSeq NM_017891.2 (NON-CURRENT)
Match location:
Position 1794 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195768)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTGGATCTCAGCTGGATTT
Hairpin Sequence:
5'-CCGG-CTCTGGATCTCAGCTGGATTT-CTCGAG-AAATCCAGCTGAGATCCAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTGGATCTCAGCTGGATTTCTCGAGAAATCCAGCTGAGATCCAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTGGATCTCAGCTGGATTTCTCGAGAAATCCAGCTGAGATCCAGAG-3'

Other clones with same target sequence:

(none)