Construct: shRNA TRCN0000195837

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146051.1-211s1c1
DNA Barcode:
CCGACTCTTGAAGGACATAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
3830406C13Rik (218734)
Gene Description:
RIKEN cDNA 3830406C13 gene
Transcript:
RefSeq NM_146051.1 (NON-CURRENT)
Match location:
Position 211 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195837)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACTCTTGAAGGACATAGA
Hairpin Sequence:
5'-CCGG-CCGACTCTTGAAGGACATAGA-CTCGAG-TCTATGTCCTTCAAGAGTCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACTCTTGAAGGACATAGACTCGAGTCTATGTCCTTCAAGAGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACTCTTGAAGGACATAGACTCGAGTCTATGTCCTTCAAGAGTCGG-3'

Other clones with same target sequence:

  1. TRCN0000345723