Construct: shRNA TRCN0000195878

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007806.1-308s1c1
DNA Barcode:
CTCACCAGGAATTACTACGTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyba (13057)
Gene Description:
cytochrome b-245, alpha polypeptide
Transcript:
RefSeq NM_007806.1 (NON-CURRENT)
Match location:
Position 308 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195878)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACCAGGAATTACTACGTC
Hairpin Sequence:
5'-CCGG-CTCACCAGGAATTACTACGTC-CTCGAG-GACGTAGTAATTCCTGGTGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACCAGGAATTACTACGTCCTCGAGGACGTAGTAATTCCTGGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACCAGGAATTACTACGTCCTCGAGGACGTAGTAATTCCTGGTGAG-3'

Other clones with same target sequence:

(none)