Construct: shRNA TRCN0000195900

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177866.2-1052s1c1
DNA Barcode:
CCCTGAGTAACCTCACAGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Catsper4 (329954)
Gene Description:
cation channel, sperm associated 4
Transcript:
RefSeq NM_177866.2 (NON-CURRENT)
Match location:
Position 1052 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195900)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGAGTAACCTCACAGAAA
Hairpin Sequence:
5'-CCGG-CCCTGAGTAACCTCACAGAAA-CTCGAG-TTTCTGTGAGGTTACTCAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGAGTAACCTCACAGAAACTCGAGTTTCTGTGAGGTTACTCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGAGTAACCTCACAGAAACTCGAGTTTCTGTGAGGTTACTCAGGG-3'

Other clones with same target sequence:

(none)