Construct: shRNA TRCN0000195926

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003917.1-1533s1c1
DNA Barcode:
CCGAGTGATTCTTGCACACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg9a (245860)
Gene Description:
autophagy related 9A
Transcript:
RefSeq NM_001003917.1 (NON-CURRENT)
Match location:
Position 1533 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195926)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTGATTCTTGCACACAT
Hairpin Sequence:
5'-CCGG-CCGAGTGATTCTTGCACACAT-CTCGAG-ATGTGTGCAAGAATCACTCGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTGATTCTTGCACACATCTCGAGATGTGTGCAAGAATCACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTGATTCTTGCACACATCTCGAGATGTGTGCAAGAATCACTCGG-3'

Other clones with same target sequence:

(none)