Construct: shRNA TRCN0000195943

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010209.1-147s1c1
DNA Barcode:
CGTGTAGAGTTCGACACCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fh1 (14194)
Gene Description:
fumarate hydratase 1
Transcript:
RefSeq NM_010209.1 (NON-CURRENT)
Match location:
Position 147 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000195943)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTAGAGTTCGACACCTTT
Hairpin Sequence:
5'-CCGG-CGTGTAGAGTTCGACACCTTT-CTCGAG-AAAGGTGTCGAACTCTACACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTAGAGTTCGACACCTTTCTCGAGAAAGGTGTCGAACTCTACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTAGAGTTCGACACCTTTCTCGAGAAAGGTGTCGAACTCTACACG-3'

Other clones with same target sequence:

(none)