Construct: shRNA TRCN0000196024
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_177897.3-736s1c2
- DNA Barcode:
- GAACTGCTCCACAAGCAAGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- B4galnt4 (330671)
- Gene Description:
- beta-1,4-N-acetyl-galactosaminyl transferase 4
- Transcript:
- RefSeq NM_177897.3 (CURRENT)
- Match location:
- Position 736 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000196024)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAACTGCTCCACAAGCAAGAT
- Hairpin Sequence:
-
5'-CCGG-GAACTGCTCCACAAGCAAGAT-CTCGAG-ATCTTGCTTGTGGAGCAGTTC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAACTGCTCCACAAGCAAGATCTCGAGATCTTGCTTGTGGAGCAGTTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAACTGCTCCACAAGCAAGATCTCGAGATCTTGCTTGTGGAGCAGTTC-3'
Other clones with same target sequence:
(none)
