Construct: shRNA TRCN0000196053

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183296.1-407s1c1
DNA Barcode:
GAATGCCTGCTCTGGAAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krtap16-3 (71369)
Gene Description:
keratin associated protein 16-3
Transcript:
RefSeq NM_183296.1 (CURRENT)
Match location:
Position 407 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196053)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATGCCTGCTCTGGAAAGAA
Hairpin Sequence:
5'-CCGG-GAATGCCTGCTCTGGAAAGAA-CTCGAG-TTCTTTCCAGAGCAGGCATTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATGCCTGCTCTGGAAAGAACTCGAGTTCTTTCCAGAGCAGGCATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATGCCTGCTCTGGAAAGAACTCGAGTTCTTTCCAGAGCAGGCATTC-3'

Other clones with same target sequence:

(none)