Construct: shRNA TRCN0000196070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001003405.1-239s1c1
DNA Barcode:
GCACAACATCAATGTCCTCGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1810049H19Rik (435889)
Gene Description:
RIKEN cDNA 1810049H19 gene
Transcript:
RefSeq NM_001003405.1 (NON-CURRENT)
Match location:
Position 239 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAACATCAATGTCCTCGA
Hairpin Sequence:
5'-CCGG-GCACAACATCAATGTCCTCGA-CTCGAG-TCGAGGACATTGATGTTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAACATCAATGTCCTCGACTCGAGTCGAGGACATTGATGTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAACATCAATGTCCTCGACTCGAGTCGAGGACATTGATGTTGTGC-3'

Other clones with same target sequence:

(none)