Construct: shRNA TRCN0000196090

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023633.1-1904s1c1
DNA Barcode:
GTCGGGTTCTTACCTTGCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Riox1 (71952)
Gene Description:
ribosomal oxygenase 1
Transcript:
RefSeq NM_023633.1 (NON-CURRENT)
Match location:
Position 1904 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196090)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGGGTTCTTACCTTGCTAA
Hairpin Sequence:
5'-CCGG-GTCGGGTTCTTACCTTGCTAA-CTCGAG-TTAGCAAGGTAAGAACCCGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGGGTTCTTACCTTGCTAACTCGAGTTAGCAAGGTAAGAACCCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGGGTTCTTACCTTGCTAACTCGAGTTAGCAAGGTAAGAACCCGAC-3'

Other clones with same target sequence:

(none)