Construct: shRNA TRCN0000196096

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153584.1-2238s1c1
DNA Barcode:
GCTGGAAATGCCTGACAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam214a (235493)
Gene Description:
family with sequence similarity 214, member A
Transcript:
RefSeq NM_153584.1 (NON-CURRENT)
Match location:
Position 2238 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196096)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAAATGCCTGACAGTTT
Hairpin Sequence:
5'-CCGG-GCTGGAAATGCCTGACAGTTT-CTCGAG-AAACTGTCAGGCATTTCCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAAATGCCTGACAGTTTCTCGAGAAACTGTCAGGCATTTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAAATGCCTGACAGTTTCTCGAGAAACTGTCAGGCATTTCCAGC-3'

Other clones with same target sequence:

(none)