Construct: shRNA TRCN0000196105
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001030274.1-269s1c1
- DNA Barcode:
- GAGTGCTTGCTTCGGTACAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ndufs5 (595136)
- Gene Description:
- NADH dehydrogenase (ubiquinone) Fe-S protein 5
- Transcript:
- RefSeq NM_001030274.1 (CURRENT)
- Match location:
- Position 269 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000196105)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GAGTGCTTGCTTCGGTACAAA
- Hairpin Sequence:
-
5'-CCGG-GAGTGCTTGCTTCGGTACAAA-CTCGAG-TTTGTACCGAAGCAAGCACTC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGAGTGCTTGCTTCGGTACAAACTCGAGTTTGTACCGAAGCAAGCACTCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGAGTGCTTGCTTCGGTACAAACTCGAGTTTGTACCGAAGCAAGCACTC-3'
Other clones with same target sequence:
-
TRCN0000041382
