Construct: shRNA TRCN0000196109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010500.1-1325s1c1
DNA Barcode:
GACCTAGAGTTGGAGGCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ier5 (15939)
Gene Description:
immediate early response 5
Transcript:
RefSeq NM_010500.1 (NON-CURRENT)
Match location:
Position 1325 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTAGAGTTGGAGGCTTAT
Hairpin Sequence:
5'-CCGG-GACCTAGAGTTGGAGGCTTAT-CTCGAG-ATAAGCCTCCAACTCTAGGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTAGAGTTGGAGGCTTATCTCGAGATAAGCCTCCAACTCTAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTAGAGTTGGAGGCTTATCTCGAGATAAGCCTCCAACTCTAGGTC-3'

Other clones with same target sequence:

(none)