Construct: shRNA TRCN0000196116

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026893.2-2439s1c1
DNA Barcode:
GCCACATCACAGCCTAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dcaf12 (68970)
Gene Description:
DDB1 and CUL4 associated factor 12
Transcript:
RefSeq NM_026893.2 (NON-CURRENT)
Match location:
Position 2439 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196116)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCACATCACAGCCTAGATAA
Hairpin Sequence:
5'-CCGG-GCCACATCACAGCCTAGATAA-CTCGAG-TTATCTAGGCTGTGATGTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCACATCACAGCCTAGATAACTCGAGTTATCTAGGCTGTGATGTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCACATCACAGCCTAGATAACTCGAGTTATCTAGGCTGTGATGTGGC-3'

Other clones with same target sequence:

(none)