Construct: shRNA TRCN0000196258

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025052.3-2112s1c1
DNA Barcode:
GCAGACGTATCACCAATAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K19 (80122)
Gene Description:
mitogen-activated protein kinase kinase kinase 19
Transcript:
RefSeq NM_025052.3 (NON-CURRENT)
Match location:
Position 2112 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196258)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACGTATCACCAATAAAT
Hairpin Sequence:
5'-CCGG-GCAGACGTATCACCAATAAAT-CTCGAG-ATTTATTGGTGATACGTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACGTATCACCAATAAATCTCGAGATTTATTGGTGATACGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACGTATCACCAATAAATCTCGAGATTTATTGGTGATACGTCTGC-3'

Other clones with same target sequence:

(none)