Construct: shRNA TRCN0000196263

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002731.2-523s1c1
DNA Barcode:
GAGCATACTTTGAATGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKACB (5567)
Gene Description:
protein kinase cAMP-activated catalytic subunit beta
Transcript:
RefSeq NM_002731.2 (NON-CURRENT)
Match location:
Position 523 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196263)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCATACTTTGAATGAGAAA
Hairpin Sequence:
5'-CCGG-GAGCATACTTTGAATGAGAAA-CTCGAG-TTTCTCATTCAAAGTATGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCATACTTTGAATGAGAAACTCGAGTTTCTCATTCAAAGTATGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCATACTTTGAATGAGAAACTCGAGTTTCTCATTCAAAGTATGCTC-3'

Other clones with same target sequence:

(none)