Construct: shRNA TRCN0000196272

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006206.3-5354s1c1
DNA Barcode:
GCTTAATTGCTGATACCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDGFRA (5156)
Gene Description:
platelet derived growth factor receptor alpha
Transcript:
RefSeq NM_006206.3 (NON-CURRENT)
Match location:
Position 5354 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196272)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTAATTGCTGATACCATAT
Hairpin Sequence:
5'-CCGG-GCTTAATTGCTGATACCATAT-CTCGAG-ATATGGTATCAGCAATTAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTAATTGCTGATACCATATCTCGAGATATGGTATCAGCAATTAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTAATTGCTGATACCATATCTCGAGATATGGTATCAGCAATTAAGC-3'

Other clones with same target sequence:

(none)