Construct: shRNA TRCN0000196405

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012290.3-1553s1c1
DNA Barcode:
GAGAGTATAGAATACACAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TLK1 (9874)
Gene Description:
tousled like kinase 1
Transcript:
RefSeq NM_012290.3 (NON-CURRENT)
Match location:
Position 1553 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196405)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGTATAGAATACACAAAG
Hairpin Sequence:
5'-CCGG-GAGAGTATAGAATACACAAAG-CTCGAG-CTTTGTGTATTCTATACTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGTATAGAATACACAAAGCTCGAGCTTTGTGTATTCTATACTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGTATAGAATACACAAAGCTCGAGCTTTGTGTATTCTATACTCTC-3'

Other clones with same target sequence:

(none)