Construct: shRNA TRCN0000196409

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015112.1-558s1c1
DNA Barcode:
GTCAGGATGATTGTAAGTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAST2 (23139)
Gene Description:
microtubule associated serine/threonine kinase 2
Transcript:
RefSeq NM_015112.1 (NON-CURRENT)
Match location:
Position 558 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196409)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGGATGATTGTAAGTTAT
Hairpin Sequence:
5'-CCGG-GTCAGGATGATTGTAAGTTAT-CTCGAG-ATAACTTACAATCATCCTGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGGATGATTGTAAGTTATCTCGAGATAACTTACAATCATCCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGGATGATTGTAAGTTATCTCGAGATAACTTACAATCATCCTGAC-3'

Other clones with same target sequence:

(none)