Construct: shRNA TRCN0000196421

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014840.2-3977s1c1
DNA Barcode:
GCTATGTAGTTGTCATCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUAK1 (9891)
Gene Description:
NUAK family kinase 1
Transcript:
RefSeq NM_014840.2 (CURRENT)
Match location:
Position 3977 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000196421)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATGTAGTTGTCATCTTTG
Hairpin Sequence:
5'-CCGG-GCTATGTAGTTGTCATCTTTG-CTCGAG-CAAAGATGACAACTACATAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATGTAGTTGTCATCTTTGCTCGAGCAAAGATGACAACTACATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATGTAGTTGTCATCTTTGCTCGAGCAAAGATGACAACTACATAGC-3'

Other clones with same target sequence:

(none)